CpGenome Universal DNA Modification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Protocol tips
Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Publication protocol

Methylation-specific PCR (MSP) and bisulfite sequencing PCR (BSP)
DNA from tissue samples and cell lines were subjected to bisulfite treatment using CpGgenome DNA Modification Kit (Chemicon, USA). Sequences, Tm, and product length of each primer used for MSP and BSP analysis are summarized in Table The band expanded with methylation-specific PCR primers corresponding to the DNA methylation in the promoter region was marked as "M". The band expanded with non-methylation-specific primers was marked as "U".



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Papers

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Manufacturer protocol

Download the product protocol from Millipore for CpGenome Universal DNA Modification Kit below.

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