Cell Lysis Buffer (10X)

Protocol tips

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	- The tissue is lysed in cell lysis buffer (Cell Signaling) supplemented with complete EDTA-free protease inhibitors, PhosSTOP phosphatase inhibitors, 1 mM PMSF and 50 mM NaF.

Protocol tips
- The tissue is lysed in cell lysis buffer (Cell Signaling) supplemented with complete EDTA-free protease inhibitors, PhosSTOP phosphatase inhibitors, 1 mM PMSF and 50 mM NaF.

Publication protocol

Whole lungs from E14.5 mice or caudal lobes from 10-week-old mice were lysed in cell lysis buffer (Cell Signaling) supplemented with complete EDTA-free protease inhibitors (Roche), PhosSTOP phosphatase inhibitors (Roche), 1 mM PMSF (Sigma-Aldrich) and 50 mM NaF (Sigma-Aldrich). Subcellular fractionation to isolate the membrane and cytoplasmic fraction was performed using a Plasma membrane Protein Extraction Kit (Abcam) following the manufacturer's instructions. Protein concentration was assessed by Bradford assay (Sigma-Aldrich) following the manufacturer's instructions.

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