Acridine Orange hemi(zinc chloride) salt

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Intercalates between DNA/RNA and used to analyze mitochondria and Lysosomal content by flow cytometry.	Stain the cells with 2 μg/ml acridine orange (AO) in phosphate buffered saline for 15 min at 37 °C and immediately analyze with a fluorescence microscope

Upstream tips
Intercalates between DNA/RNA and used to analyze mitochondria and Lysosomal content by flow cytometry.
Protocol tips
Stain the cells with 2 μg/ml acridine orange (AO) in phosphate buffered saline for 15 min at 37 °C and immediately analyze with a fluorescence microscope

Publication protocol

GL15 cells were grown in glass coverslips for 3 days, then serum starved for 48 hours in the presence or absence of 400 μM LND. The cells were stained with 2 μg/ml acridine orange (AO) in phosphate buffered saline for 15 min at 37 °C and immediately analyzed with a fluorescence microscope (DMRB Leika).

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Manufacturer protocol

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