Gibco™Neurobasal™ Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Culture vessels were coated with poly-l-lysine.	Neurobasal medium (ThermoFisher, 21,103), B-27 supplement (ThermoFisher, 17,504), GlutaMax-I supplement (ThermoFisher, 35,050), dibutyryl cAMP (0.5 mM added on day 7 for three days) and primocin was used for NSCs.

Upstream tips
Culture vessels were coated with poly-l-lysine.
Protocol tips
Neurobasal medium (ThermoFisher, 21,103), B-27 supplement (ThermoFisher, 17,504), GlutaMax-I supplement (ThermoFisher, 35,050), dibutyryl cAMP (0.5 mM added on day 7 for three days) and primocin was used for NSCs.

Publication protocol

Neurobasal medium (ThermoFisher, 21,103), B-27 supplement (ThermoFisher, 17,504), GlutaMax-I supplement (ThermoFisher, 35,050), dibutyryl cAMP (0.5 mM added on day 7 for three days) and primocin was used for NSCs.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™Neurobasal™ Medium below.

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