EZ DNA Methylation kit

DNA methylation profiling Whole genome profiling - mouse liver tissue

Experiment
DNA methylation profiling Whole genome profiling - mouse liver tissue
Product
EZ DNA Methylation kit from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Bisulfite sequencing and combined bisulfite restriction analysis (COBRA)
500 ng of genomic DNA from each mouse liver tissue was treated with bisulfite using EZ DNA Methylation-Gold Kit (Zymo Research, Orange, CA, USA) followed by PCR. The primers used were listed in . For bisulfite sequencing, the PCR products of three control or TCE treated samples were pooled and cloned into TA-cloning vector (Tiangen), and individual clones were sequenced using an automatic sequencer (ABI 3730XL). For COBRA, the PCR products were digested with the corresponding restriction enzymes and analyzed on 2% agarose gel as described earlier .

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Papers

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Paper title
Trichloroethylene-Induced Gene Expression and DNA Methylation Changes in B6C3F1 Mouse Liver
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Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

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