Discussion

2 months ago

S Janardhan Belgium

2

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0

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Reactivate dormant Lactobacillus Paracasei

Hello fellow Labettors, I would like to know what would be the best method/medium to reactivate my dormant Lactobacillus paracasei cells?. Any suggestions are greatly appreciated.

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Discussion

3 months ago

Ralf Friedmann Switzerland

2

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1

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I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Experiment:

Discussion

3 months ago

Jean Rossignol France

1

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1

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How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

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Discussion

2 months ago

0

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0

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Multiplex PCR and qPCR

I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?

Experiment:

Discussion

3 months ago

Nicolai Brandt Denmark

1

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1

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What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Discussion

2 months ago

Vitoria Rocha Almeida Brazil

0

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0

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Will I be able to ligate blunt ends without the presence of ATP?

Will I be able to ligate blunt ends without the presence of ATP?

Experiment:

Discussion

3 months ago

Myrle Houtzager Netherlands

0

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1

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Can I use the same reagent to transfect a different protein in the same cell line?

Will the reagent that I used to transfect a protein, work for a different protein that I want to transfect in the same cell line?

Experiment:

Discussion

3 months ago

Israel Lev Israel

1

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1

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How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Experiment:

Discussion

3 months ago

Eufrosina Sagese Italy

1

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1

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Is a knockdown using shRNA permanent?

Is a knockdown using shRNA permanent and if not is there a known duration?

Experiment:

Discussion

3 months ago

Maryam Amir Qatar

2

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0

Comments

When should I add the polymerase for hot start PCR?

I keep getting a non-specific band in PCR so I would like to try a hot start PCR manually. How should I prepare the mix and at which step should I be adding the polymerase?

Experiment:

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