Discussion

4 years ago

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Experiment: DNA isolation / purification Bacteria - Gram negative Legionella

Top comment


Hi Israel, Interesting question indeed!

Answered 4 years ago

Dhr. L.S. Petitiaux

2 Comments


Start with more cells or run multiple purifications and combine the results. If this is plasmid DNA, be sure to choose a high copy form. Remember to rely heavily on cold precipitation temps as well. Follow the time events in your recipe accurately.

Answered 3 years ago


Hi Israel, Interesting question indeed!

Answered 4 years ago

Dhr. L.S. Petitiaux

Can you help?

Similar discussions


Start your discussion


Share your thoughts or question with experts in your field

Start discussion
Become shareholder Discussions About us Contact Privacy Terms