Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Proteins ChIP Anti-bodies GATA3 ChIP Anti-bodies GATA3 In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody. 2 Matching solutions Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 2 matching solutions for this experiment GATA-3 Antibody (HG3-31): sc-268 Santa Cruz Biotechnology Upstream tips Protocol tips Downstream tips -Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases. Protocol tips -Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases. Manufacturer protocol Publication protocol 1 Relevant paper Recombinant Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428) Abcam Upstream tips Protocol tips Downstream tips -Add protease inhibitors to all lysis solutions before use. -Use 2 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Upstream tips -Add protease inhibitors to all lysis solutions before use. Protocol tips -Use 2 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Proteins ChIP Anti-bodies Sall4 ChIP Anti-bodies Sall4 In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody. 2 Matching solutions Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 2 matching solutions for this experiment Sall4 Antibody (EE-30): sc-101147 Santa Cruz Biotechnology Upstream tips Protocol tips Downstream tips -Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases. Protocol tips -Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases. Manufacturer protocol Publication protocol 1 Relevant paper Anti-Sall4 antibody - ChIP Grade (ab29112) Abcam Upstream tips Protocol tips Downstream tips -Add protease inhibitors to all lysis solutions before use. -Use at an assay dependent concentration. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Upstream tips -Add protease inhibitors to all lysis solutions before use. Protocol tips -Use at an assay dependent concentration. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Proteins ChIP Anti-bodies TFIIB ChIP Anti-bodies TFIIB In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment TFIIB Antibody (D-3): sc-271736 Santa Cruz Biotechnology Upstream tips Protocol tips Downstream tips -Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases. Protocol tips -Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Proteins ChIP Anti-bodies Stat5b ChIP Anti-bodies Stat5b In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody. 2 Matching solutions Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 2 matching solutions for this experiment Stat5b Antibody (C-8): sc-377069 Santa Cruz Biotechnology Upstream tips Protocol tips Downstream tips -Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases. Protocol tips -Investigators may wish to consider using the primary antibody conjugated to sepharose or magnetic beads as an alternative to using secondary immunoprecipitation reagents (e.g., Protein A-Agarose) as described here. Combining primary antibodies directed to different epitopes of the same protein may be advantageous in some cases. Manufacturer protocol Publication protocol 1 Relevant paper STAT5 beta Monoclonal Antibody (ST5b-10G1) Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips -Tested Dilution 5 µg/mL Protocol tips -Tested Dilution 5 µg/mL Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Proteins ChIP Anti-bodies FLAG ChIP Anti-bodies FLAG In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody. 2 Matching solutions Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 2 matching solutions for this experiment DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 Cell Signaling Technology Upstream tips Protocol tips Downstream tips -It is highly critical that the chromatin is of appropriate size and concentration. -For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. -It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date. -Once in solution, store 1M DTT at -20°C. Upstream tips -It is highly critical that the chromatin is of appropriate size and concentration. Protocol tips -For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. -It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date. Downstream tips -Once in solution, store 1M DTT at -20°C. Manufacturer protocol Publication protocol 1 Relevant paper Monoclonal ANTI-FLAG® M2 antibody produced in mouse Sigma-Aldrich Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Proteins ChIP Anti-bodies CTCF ChIP Anti-bodies CTCF In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody. 3 Matching solutions Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 3 matching solutions for this experiment CTCF (D31H2) XP® Rabbit mAb #3418 Cell Signaling Technology Upstream tips Protocol tips Downstream tips -It is highly critical that the chromatin is of appropriate size and concentration. -For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. -It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date. -Once in solution, store 1M DTT at -20°C. Upstream tips -It is highly critical that the chromatin is of appropriate size and concentration. Protocol tips -For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. -It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date. Downstream tips -Once in solution, store 1M DTT at -20°C. Manufacturer protocol Publication protocol 1 Relevant paper Anti-CTCF antibody - ChIP Grade (ab70303) Abcam Upstream tips Protocol tips Downstream tips -Add protease inhibitors to all lysis solutions before use. -Use at an assay dependent concentration. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Upstream tips -Add protease inhibitors to all lysis solutions before use. Protocol tips -Use at an assay dependent concentration. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Manufacturer protocol Publication protocol 1 Relevant paper Anti-CTCF Antibody Merck Millipore Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Proteins ChIP Anti-bodies HDAC1 ChIP Anti-bodies HDAC1 In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody. 3 Matching solutions Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 3 matching solutions for this experiment HDAC1 (D5C6U) XP® Rabbit mAb #34589 Cell Signaling Technology Upstream tips Protocol tips Downstream tips -It is highly critical that the chromatin is of appropriate size and concentration. -For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. -It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date. -Once in solution, store 1M DTT at -20°C. Upstream tips -It is highly critical that the chromatin is of appropriate size and concentration. Protocol tips -For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. -It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date. Downstream tips -Once in solution, store 1M DTT at -20°C. Manufacturer protocol Publication protocol 1 Relevant paper Anti-HDAC1 antibody - ChIP Grade (ab7028) Abcam Upstream tips Protocol tips Downstream tips -Add protease inhibitors to all lysis solutions before use. -Use 2 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Upstream tips -Add protease inhibitors to all lysis solutions before use. Protocol tips -Use 2 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Manufacturer protocol Publication protocol 1 Relevant paper ChIPAb+™ HDAC1 Antibody, rabbit polyclonal Merck Millipore Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Proteins ChIP Anti-bodies H3.3 ChIP Anti-bodies H3.3 In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment Recombinant Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade (ab176840) Abcam Upstream tips Protocol tips Downstream tips -Add protease inhibitors to all lysis solutions before use. -Use 2 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Upstream tips -Add protease inhibitors to all lysis solutions before use. Protocol tips -Use 2 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Proteins ChIP Anti-bodies MRE11 ChIP Anti-bodies MRE11 In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody. 2 Matching solutions Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 2 matching solutions for this experiment Mre11 Antibody #4895 Cell Signaling Technology Upstream tips Protocol tips Downstream tips -It is highly critical that the chromatin is of appropriate size and concentration. -It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date. -Once in solution, store 1M DTT at -20°C. Upstream tips -It is highly critical that the chromatin is of appropriate size and concentration. Protocol tips -It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date. Downstream tips -Once in solution, store 1M DTT at -20°C. Manufacturer protocol Publication protocol 1 Relevant paper Anti-Mre11 antibody - ChIP Grade (ab12159) Abcam Upstream tips Protocol tips Downstream tips -Add protease inhibitors to all lysis solutions before use. -Use at an assay dependent dilution. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Upstream tips -Add protease inhibitors to all lysis solutions before use. Protocol tips -Use at an assay dependent dilution. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Manufacturer protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Proteins ChIP Anti-bodies H3K27me3 ChIP Anti-bodies H3K27me3 In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody. 3 Matching solutions Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 3 matching solutions for this experiment Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 Cell Signaling Technology Upstream tips Protocol tips Downstream tips -It is highly critical that the chromatin is of appropriate size and concentration. -For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. -It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date. -Once in solution, store 1M DTT at -20°C. Upstream tips -It is highly critical that the chromatin is of appropriate size and concentration. Protocol tips -For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. -It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date. Downstream tips -Once in solution, store 1M DTT at -20°C. Manufacturer protocol Publication protocol 1 Relevant paper Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) Abcam Upstream tips Protocol tips Downstream tips -Add protease inhibitors to all lysis solutions before use. -Use 2 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Upstream tips -Add protease inhibitors to all lysis solutions before use. Protocol tips -Use 2 µg for 25 µg of chromatin. -Keep cells on ice between the rounds of homogenisations. - Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line. Manufacturer protocol Publication protocol 1 Relevant paper Anti-trimethyl-Histone H3 (Lys27) Antibody Millipore Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms