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PCR Multiplex PCR

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PCR Multiplex PCR - Bacterial DNA Experiment

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

DNA PCR Multiplex PCR Bacterial DNA
PCR Multiplex PCR - Mammalian DNA Experiment

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

DNA PCR Multiplex PCR Mammalian DNA
QuantiFast Multiplex PCR Kit Product

Get tips on using QuantiFast Multiplex PCR Kit to perform PCR Multiplex PCR - Bacterial DNA

Products Qiagen QuantiFast Multiplex PCR Kit
QIAGEN Multiplex PCR Kit Product

Get tips on using QIAGEN Multiplex PCR Kit to perform PCR Multiplex PCR - Mammalian DNA

Products Qiagen QIAGEN Multiplex PCR Kit
PCR Multiplex PCR - Viral Experiment

DNA PCR Multiplex PCR Viral
QuantiTect Multiplex PCR Kit (1000) Product

Get tips on using QuantiTect Multiplex PCR Kit (1000) to perform PCR Multiplex PCR - Bacterial DNA

Products Qiagen QuantiTect Multiplex PCR Kit (1000)
QuantiFast Multiplex PCR Kit Product

Get tips on using QuantiFast Multiplex PCR Kit to perform PCR Quantitative real-time PCR - Bacterial DNA

Products Qiagen QuantiFast Multiplex PCR Kit
PCR Multiplex PCR - Poultry DNA Experiment

DNA PCR Multiplex PCR Poultry DNA
QuantiNova Multiplex RT-PCR Kit (2500) Product

Get tips on using QuantiNova Multiplex RT-PCR Kit (2500) to perform PCR Multiplex PCR - Viral

Products Qiagen QuantiNova Multiplex RT-PCR Kit (2500)
QuantiTect Multiplex RT-PCR Kit (1000) Product

Get tips on using QuantiTect Multiplex RT-PCR Kit (1000) to perform PCR Multiplex PCR - Viral

Products Qiagen QuantiTect Multiplex RT-PCR Kit (1000)

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