Found 555 results for siRNA / RNAi /miRNA transfection.


Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / RNAi /miRNA transfection Human HeLa Lipofectamine siRNA / RNAi /miRNA transfection Human - HeLa Lipofectamine The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to be considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by Keith L. Morrison siRNA/RNAi/miRNA transfection human I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method? Comment View 2 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment Lipofectamine® 2000 Transfection Reagent Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1–3 days at 37°C. Protocol tips Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1–3 days at 37°C. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

RNA siRNA / RNAi /miRNA transfection Human HeLa Lipofectamine

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / RNAi /miRNA transfection Human KG-1 cells Lipofectamine siRNA / RNAi /miRNA transfection Human - KG-1 cells Lipofectamine The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by Keith L. Morrison siRNA/RNAi/miRNA transfection human I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method? Comment View 2 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment Lipofectamine® RNAiMAX Transfection Reagent Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips siRNA concentration- 30 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).  Incubate for 5 minutes at room temperature and add to cells.  Incubate cells for 3 days at 37°C.  Protocol tips siRNA concentration- 30 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).  Incubate for 5 minutes at room temperature and add to cells.  Incubate cells for 3 days at 37°C.  Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

RNA siRNA / RNAi /miRNA transfection Human KG-1 cells Lipofectamine

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / RNAi /miRNA transfection Human Jurkat cells Lipofectamine siRNA / RNAi /miRNA transfection Human - Jurkat cells Lipofectamine The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by Keith L. Morrison siRNA/RNAi/miRNA transfection human I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method? Comment View 2 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment Lipofectamine® RNAiMAX Transfection Reagent Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips siRNA sequence:sequences are 5′-CAUCUCCUAUGGCGGUGGU (siNB1i) and 5′-UGGUCCUCCUCAAGGCAGU (siNB2i) Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).  Incubate for 5 minutes at room temperature and add to cells.  Incubate cells for 3 days at 37°C.  Protocol tips siRNA sequence:sequences are 5′-CAUCUCCUAUGGCGGUGGU (siNB1i) and 5′-UGGUCCUCCUCAAGGCAGU (siNB2i) Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).  Incubate for 5 minutes at room temperature and add to cells.  Incubate cells for 3 days at 37°C.  Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

RNA siRNA / RNAi /miRNA transfection Human Jurkat cells Lipofectamine

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / RNAi /miRNA transfection Human Primary splenocytes Polymer / lipid siRNA / RNAi /miRNA transfection Human - Primary splenocytes Polymer / lipid The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by Keith L. Morrison siRNA/RNAi/miRNA transfection human I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method? Comment View 2 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment TransIT-TKO Transfection Reagent Mirus Upstream tips Protocol tips Downstream tips siRNA concentration-50–100 nM final concentration Mix siRNA:reagent mixture and incubate at room temperature for 15–30 minutes. Add mixture to cells and incubate for 24hours. Protocol tips siRNA concentration-50–100 nM final concentration Mix siRNA:reagent mixture and incubate at room temperature for 15–30 minutes. Add mixture to cells and incubate for 24hours. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

RNA siRNA / RNAi /miRNA transfection Human Primary splenocytes Polymer / lipid

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / RNAi /miRNA transfection Human NK92 cells Lipofectamine siRNA / RNAi /miRNA transfection Human - NK92 cells Lipofectamine The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by Keith L. Morrison siRNA/RNAi/miRNA transfection human I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method? Comment View 2 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment Lipofectamine® RNAiMAX Transfection Reagent Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 min at RT. Incubate cells for 24-72 h at 37°C. Protocol tips Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 min at RT. Incubate cells for 24-72 h at 37°C. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

RNA siRNA / RNAi /miRNA transfection Human NK92 cells Lipofectamine

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / RNAi /miRNA transfection Human THP-1 cells Lipofectamine siRNA / RNAi /miRNA transfection Human - THP-1 cells Lipofectamine The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by Keith L. Morrison siRNA/RNAi/miRNA transfection human I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method? Comment View 2 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment Lipofectamine® RNAiMAX Transfection Reagent Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).  Incubate for 5 minutes at room temperature and add to cells.  Incubate cells for 1 day at 37°C.  Protocol tips Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).  Incubate for 5 minutes at room temperature and add to cells.  Incubate cells for 1 day at 37°C.  Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

RNA siRNA / RNAi /miRNA transfection Human THP-1 cells Lipofectamine

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / RNAi /miRNA transfection Human Human Endometrial Stromal Cells Lipofectamine siRNA / RNAi /miRNA transfection Human - Human Endometrial Stromal Cells Lipofectamine The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by Keith L. Morrison siRNA/RNAi/miRNA transfection human I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method? Comment View 2 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment Lipofectamine® 2000 Transfection Reagent Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature. Incubate cells for 18h at 37°C and change medium and re-incubate for 72 hours Protocol tips Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature. Incubate cells for 18h at 37°C and change medium and re-incubate for 72 hours Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

RNA siRNA / RNAi /miRNA transfection Human Human Endometrial Stromal Cells Lipofectamine

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / RNAi /miRNA transfection Human Ovarian cancer OV2008 cells Lipofectamine siRNA / RNAi /miRNA transfection Human - Ovarian cancer OV2008 cells Lipofectamine The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by Keith L. Morrison siRNA/RNAi/miRNA transfection human I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method? Comment View 2 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment Lipofectamine® RNAiMAX Transfection Reagent Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips siRNA concentration of 50 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells fro 1-3 days at 37C Protocol tips siRNA concentration of 50 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells fro 1-3 days at 37C Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

RNA siRNA / RNAi /miRNA transfection Human Ovarian cancer OV2008 cells Lipofectamine

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / RNAi /miRNA transfection Human Cal 27 cells Polymer / lipid siRNA / RNAi /miRNA transfection Human - Cal 27 cells Polymer / lipid The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by Keith L. Morrison siRNA/RNAi/miRNA transfection human I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method? Comment View 2 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment TransIT®-LT1 Transfection Reagent Mirus Upstream tips Protocol tips Downstream tips Incubate TransIT-LT1 Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells.  Incubate for 72 hours Protocol tips Incubate TransIT-LT1 Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells.  Incubate for 72 hours Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

RNA siRNA / RNAi /miRNA transfection Human Cal 27 cells Polymer / lipid

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2) Lipofectamine siRNA / RNAi /miRNA transfection Human - Lung Adenocarcenoma (A549/LTEP-a-2) Lipofectamine The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by Keith L. Morrison siRNA/RNAi/miRNA transfection human I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method? Comment View 2 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment Lipofectamine® 2000 Transfection Reagent Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1–3 days at 37°C. Protocol tips Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1–3 days at 37°C. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

RNA siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2) Lipofectamine
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