Found 604 results for Acid phosphatase assay.


WST-1 assay kit - confocal microscope (LSCM, Zeiss LSM 510 META). Alkaline phosphatase activity Alkaline phosphatase activity was assayed according to the method described by Lowry et al. In brief, to lyse cells the matrices were washed with PBS and then suspended in 0.5 mL PBS containing 0.1 M glycine, 1 mM MgCl

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Nucleic acid isolation and quantification - , the extraction process involved rehydration of the homogenized tissue, followed by protease digestion, binding to a solid substrate, washing, and elution, with variations specific to each kit/protocol. Three technical replicates were performed for each extraction kit. Inhibition assays During nucleic acid

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Live / Dead assay bacteria - Porphyromonas gingivalis - Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Live / Dead assay bacteria - Porphyromonas gingivalis LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy Live / Dead assay bacteria

Products Thermo Fisher Scientific LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy

Nucleic acid extraction - on the ABI 7500 Fast instrument (Applied Biosystems, Paisley, UK). mRT-PCR assays were carried out in a total volume of 20 μL, comprising 10 μL of QuantiFast Multiplex PCR mastermix (Qiagen), 2 μL of nuclease-free water (Promega), 2 μL of oligonucleotide mixture (), and 6 μL of nucleic acid extract

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Luciferase assay - phosphatase fusion proteins NELL1 protein fused to an alkaline phosphatase (AP) tag (NELL1-AP), NELL2-AP, and AP proteins were prepared and purified as previously described. Cell migration assay The cell migration assay was carried out basically as previously described. The lower side

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(iv) Acid-induced inactivation assay in BHK-21 cells. - plaque reduction of the mixtures was determined by a plaque-forming assay on BHK-21 cell monolayers (, ). (iv) Acid-induced inactivation assay in BHK-21 cells. Portions (10 μl) of the indicated virus stocks (0.5 multiplicity of infection [MOI]) were mixed with 300 μl of PBS solutions (50 mM NaHPO and 140

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Autophagy assay cell type - THP 1 - % deoxycholic acid, 0.1% SDS 1% Triton X-100, protease and phosphatase inhibitors Dilute primary Ab at 1:1,000 and incubate overnight at 4C. Upstream tips Lyse cells in in a modified RIPA buffer containing 150 mM NaCl, 1% NP-40, 50 mM Tris-HCl, pH 8, 0.5% deoxycholic acid, 0.1% SDS 1% Triton X-100

Products Cell Signaling Technology SAPK/JNK Antibody

Autophagy assay cell type - THP 1 - % SDS 1% Triton X-100, protease and phosphatase inhibitors Dilute primary Ab at 1:1,000 and incubate overnight at 4C. Upstream tips Lyse cells in buffer containing 150 mM NaCl, 1% NP-40, 50 mM Tris-HCl, pH 8, 0.5% deoxycholic acid, 0.1% SDS 1% Triton X-100, protease and phosphatase inhibitors Protocol

Products Cell Signaling Technology Beclin-1 Antibody

Autophagy assay cell type - THP 1 - cells in in a modified RIPA buffer containing 150 mM NaCl, 1% NP-40, 50 mM Tris-HCl, pH 8, 0.5% deoxycholic acid, 0.1% SDS 1% Triton X-100, protease and phosphatase inhibitors Dilute primary Ab at 1:1,000 and incubate overnight at 4C. Upstream tips Lyse cells in in a modified RIPA buffer containing

Products Cell Signaling Technology Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb

TUNEL assay cell type - Rabbit synovial fibroblasts - Toggle navigation Experiments Products Discussions   Login   Join for free Labettor TUNEL assay cell type - Rabbit synovial fibroblasts APO-BRDU™ Kit (RUO) APO-BRDU™ Kit (RUO) TUNEL assay cell type - Rabbit synovial fibroblasts Experiment TUNEL assay cell type - Rabbit synovial fibroblasts Product

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