Found 604 results for Acid phosphatase assay.
Autophagy assay cell type - THP 1 - Downstream tips Lyse cells in buffer containing 150 mM NaCl, 1% NP-40, 50 mM Tris-HCl, pH 8, 0.5% deoxycholic acid, 0.1% SDS 1% Triton X-100, protease and phosphatase inhibitors Incubate primary Ab overnight at 4C. Upstream tips Lyse cells in buffer containing 150 mM NaCl, 1% NP-40, 50 mM Tris-HCl, pH 8
Ribonucleic Acid Isolation and Reverse Transcription - to the supplier’s protocol (Invitrogen, Carlsbad, CA, USA). A single cDNA preparation from each specimen was used for the assay of all antimicrobial products tested. View full paper Reviews SV Total RNA Isolation System from Promega has not yet been reviewed for this experiment We'd love it if you would
Autophagy assay cell type - REH - ), as described in Results and Figure legends. Cell viability determination Cell viability was determined by measuring the cellular acid phosphatase activity . At the end of the incubation period (24 or 48 h), culture medium was removed and the acid phosphatase substrate p-nitrophenyl phosphate (10
Autophagy assay cell type - THP 1 - Upstream tips Protocol tips Downstream tips Lyse cells in in a modified RIPA buffer containing 150 mM NaCl, 1% NP-40, 50 mM Tris-HCl, pH 8, 0.5% deoxycholic acid, 0.1% SDS 1% Triton X-100, protease and phosphatase inhibitors Dilute primary Ab at 1:1,000 and incubate overnight at 4C. Upstream tips
Adipocyte differentiation assay - Manual. For fatty acid oxidation, the assay medium used was KRB buffer consisting of 110 mM NaCl, 4.7 mM KCl, 2 mM MgSO, 1.2 mM NaHPO, 2.5 mM glucose adjusted to pH 7.4 supplemented with 0.5 mM carnitine. For induction of fatty acid oxidation, palmitate-BSA complex was injected to a final concentration
Muse® Cell Cycle Assay Kit - field Start a discussion Papers Check out relevant papers found by Labettor's AI that are relevant for performing Cell cycle assay human - HaCaT using Muse® Cell Cycle Assay Kit from Merck Millipore. Paper title Lichen-derived caperatic acid and physodic acid inhibit Wnt signaling in colorectal
Nucleic acid isolation - placed in liquid nitrogen immediately after resection and stored at -80°C until use. The malignant and normal diagnosis was performed by pathologists. The study was performed after approval by our institute Medical Ethics Committee. Nucleic acid isolation The EZNA Tissue DNA Kit (Omega Corp, USA
Mammalian beta-Galactosidase Assay Kit - ). For lysosomal acid phosphatase (LAP) activity, CMs were incubated with LysoLive Phosgreen substrate (Marker Gene Technologies, Eugene, OR). Images were acquired as described in the supplementary Methods section. The lysosomal fraction described earlier was used to measure β-galactosidase activity using
Autophagy assay cell type - NRK - Autophagy assay cell type - NRK using Anti-LC3 pAb from MBL international corporation. Paper title The general amino acid control pathway regulates mTOR and autophagy during serum/glutamine starvation First author Rui Chen Materials and methods Reagents and antibodies Reagents were obtained from
Isolation and Identification of Lactic Acid Bacteria from Fermented Milk - of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA Publication protocol solation and Identification of Lactic Acid Bacteria from Fermented Milk Sampling was done from raw cow milk randomly collected from two different restaurants (Al-Berkat
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