Found 602 results for Acid phosphatase assay.

Autophagy assay cell type - HepG2 - primary Ab at 1:1000 and incubate overnight at 4°C Publication protocol The cells were lysed in radioimmunoprecipitation assay buffer containing phosphatase inhibitor cocktail I (Sigma-Aldrich; Merck KGaA) and a protease inhibitor cocktail mini-tablet (Roche Diagnostics GmbH, Mannheim, Germany

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Alkaline phosphatase and matrix mineralization assays - differentiation was induced by with 50 μM L-ascorbate-2-phosphate and 10 mM beta-glycerophosphate. Alkaline phosphatase and matrix mineralization assays Quantitative AP and matrix mineralization assays were performed as previously described []. Shortly, AP activity was determined by homogenizing 3

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fluoresce and cause background. Publication protocol Cells were harvested using RIPA (radio immunoprecipitation assay) buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% Nonidet-P40, 0.5% sodium deoxycholate and 0.5% SDS) supplemented with phosphatase (Na3VO4, 1:100, NaF, 1:200 or phosphatase inhibitor

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Autophagy assay cell type - MEFs (mouse embryonic fibroblasts) - ) or alternatively buffer B (0.05 M Tris-HCl. pH 8.0, 0.15 M NaCl, 5.0 mM EDTA, and 1% NP-40) with protease and phosphatase inhibitor cocktail tablets (Roche). Whole cell lysates were separated by SDS-PAGE and transferred onto polyvinyllidene difluoride membranes. X-peptide pulldown assay Plasmids

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Cytotoxicity LDH Assay Kit-WST - Blot Treated cells were lysed in ice-cold radio-immunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitors (phenylmethylsulfonyl fluoride, PMSF) (Beyotime, Shanghai, China). The total protein was measured using a bicinchoninic acid protein assay (BCA, Beyotime, Shanghai

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Autophagy assay cell type - A375 (human melanoma cell line) - and phosphatase inhibitors (Roche, Basel, Switzerland). The protein concentration was measured by the BCA protein assay kit (Fudebio, Hangzhou, China). The protocol we used was that same with our previous publication [38]. View full paper Reviews Beclin 1 Rabbit Polyclonal antibody from Proteintech

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Cell cycle assay human - HaCaT - with 200 mg/mL RNase A and 50 μg/mL PI Manufacturer protocol Publication protocol 1 Relevant paper Muse® Cell Cycle Assay Kit Merck Millipore Upstream tips Protocol tips Downstream tips - Following seeing, cells were treated with PKF118-310, caperatic acid, physodic acid or camptothecin. - At −20 °C

Cellular assays Cell cycle assay human HaCaT

Autophagy assay cell type - TPC 1 - protocol were lysed in RIPA (150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS, 50 mM Tris pH 8.0, and 2 mM MgCl2). Protease and phosphatase inhibitors (protease inhibitor cocktail plus 5 mM sodium fluoride, 0.5 mM sodium orthovanadate, 1 mM sodium molybdate, 50 mM 2-chloroacetamide, 2 mM 1,10

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Autophagy assay cell type - SH-SY5Y - ). For detection of phosphoproteins, SH-SY5Y cells were lysed in ice-cold buffer containing 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2 mM EDTA, 2 mM EGTA, 1% Triton, 1 nM okadaic acid, protease (code P8349, Sigma-Aldrich) and phosphatase (code 524625, Calbiochem) inhibitor cocktails. Lysates were centrifuged

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Cell cycle assay human - BxPC-3 - a discussion Papers Check out relevant papers found by Labettor's AI that are relevant for performing Cell cycle assay human - BxPC-3 using Cell Cycle and Apoptosis Analysis Kit from Beyotime. Paper title Gambogic acid sensitizes gemcitabine efficacy in pancreatic cancer by reducing the expression

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