Found 602 results for Acid phosphatase assay.


Autophagy assay cell type - INS-1E - Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Cellular assays Autophagy assay cell type INS-1E Autophagy assay cell type - INS-1E As autophagy

Cellular assays Autophagy assay cell type INS-1E

Autophagy assay cell type - NRK-52E - , and 1% phosphatase I and II inhibitor cocktail (Sigma, St Louis, MO) on ice. The supernatants were collected after centrifugation at 13,000 × g at 4 °C for 30 min. Protein concentration was determined by bicinchoninic acid protein assay. An equal amount of protein was loaded into 10% or 15% SDS

Products Sigma-Aldrich Anti-LC3B antibody produced in rabbit

Autophagy assay cell type - AML12 - A, propidium iodide (PI), (4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), dexamethasone, phosphatase and protease inhibitor cocktails, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA

Products Enzo Life Sciences CYTO-ID® Autophagy detection kit

Autophagy assay cell type - SH-SY5Y - Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Autophagy assay cell type - SH-SY5Y Purified Mouse Anti-p62 Ick ligand Clone 3/P62 LCK LIGAND (RUO) Purified Mouse Anti-p62 Ick ligand Clone 3/P62 LCK LIGAND (RUO) Autophagy assay cell type - SH-SY5Y Experiment

Products BD Biosciences Purified Mouse Anti-p62 Ick ligand Clone 3/P62 LCK LIGAND (RUO)

Autophagy assay cell type - A7r5 - cultures were lysed with RIPA lysis buffer (Tris-HCl 10 mmol/L, EDTA 5 mmol/L, NaCl 50 mmol/L, 1% deoxycholic acid and 1% triton X-100, pH 7.4). Protein concentration was determined by the Bradford method (BioRad protein assay). Equal amounts of proteins from cell extracts were separated by SDS-PAGE 8–15

Products Cell Signaling Technology Beclin-1 Antibody

Autophagy assay cell type - A2780 - Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Autophagy assay cell type - A2780 SQSTM1/p62 (D5E2) Rabbit mAb SQSTM1/p62 (D5E2) Rabbit mAb Autophagy assay cell type - A2780 Experiment Autophagy assay cell type - A2780 Product SQSTM1/p62 (D5E2) Rabbit mAb from

Products Cell Signaling Technology SQSTM1/p62 (D5E2) Rabbit mAb

and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied

Proteins Protein isolation Mammalian cells Human monocytic cell line_THP-1

OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) - previously (21). ROS production was detected using the NBT assay (22). At day 8 after induction, the cells were incubated for 90 min in PBS containing 0.2% NBT. The dark-blue formazan was dissolved in 50% acetic acid, and its absorbance was determined at 570 nm. To monitor ROS production in mature

Products Cell Biolabs OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

assay lysis buffer containing a protease and phosphatase inhibitor cocktail on ice for 30 min. Then lysate was then collected and centrifuged at 56,000 × g for 15 min at 4°C. Protein concentration was determined using the Bicinchoninic Acid Protein Quantification kit (Pierce Biotechnology, Inc

Products Proteintech Group Cyclin E1 Antibody

Autophagy assay cell type - SH-SY5Y - Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Autophagy assay cell type - SH-SY5Y LAMP1 (C54H11) Rabbit mAb LAMP1 (C54H11) Rabbit mAb Autophagy assay cell type - SH-SY5Y Experiment Autophagy assay cell type - SH-SY5Y Product LAMP1 (C54H11) Rabbit mAb from Cell

Products Cell Signaling Technology LAMP1 (C54H11) Rabbit mAb
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