No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Use of pre-warmed 24-well plate
Add fresh medium was every 2-3 days. |
Collect organoids to fix them in 4% paraformaldehyde for 1hr, embed in 2% agarose gel, or directly fix in the matrigel in formalin for generation of paraffin blocks, sectioning and staining |
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Use of pre-warmed 24-well plate
Add fresh medium was every 2-3 days. |
| Downstream tips |
| Collect organoids to fix them in 4% paraformaldehyde for 1hr, embed in 2% agarose gel, or directly fix in the matrigel in formalin for generation of paraffin blocks, sectioning and staining |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Harvest organoids after 4–7 days of growth |
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
| Harvest organoids after 4–7 days of growth |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Change medium every 4 days
Organoid passage every 2–3 days
|
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Change medium every 4 days
Organoid passage every 2–3 days
|
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!