3D Cell Culture Media Human liver organoids

Start discussion

Found 1 discussion for this experiment

Discussion

2 years ago

2 years ago by Alexya Azhakesan

Impact of using FBS

I have tried to fabricate Liver organoids and would like to study the impact of FBS on healthy and tumor organoids. Since the compositions of FBS is unknown, do you recommend any alternatives like Human platelet lysate, etc?

Comment
Share your thoughts or question with experts in your field by adding a discussion!

Found 2 matching solutions for this experiment

Gibco™Advanced DMEM/F-12 1

Thermo Fisher Scientific

Upstream tips
Defrost in advance the BME gel before use
Protocol tips
Split primary organoids after 14 days and for maintenance every 7-10 days
Manufacturer protocol Publication protocol 1 Relevant paper
DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Cultrex growth factor reduced BME type 2 in advance, on ice or in fridge
Manufacturer protocol Publication protocol 1 Relevant paper
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms