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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Seeding of ciliary ducts or sorted cells |
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
| Seeding of ciliary ducts or sorted cells |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the growth factor reduced Matrigel in advance, on ice or in fridge |
Seed 1000 cells per 20μL of matrigel droplets for primary cultures and 500 – 2000 cells per 20 μL drops for passage |
|
| Upstream tips |
| Defrost the growth factor reduced Matrigel in advance, on ice or in fridge |
| Protocol tips |
| Seed 1000 cells per 20μL of matrigel droplets for primary cultures and 500 – 2000 cells per 20 μL drops for passage |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the growth factor reduced Matrigel in advance, on ice or in fridge |
Replace growth factors every other day and Add fresh media every four days. |
|
| Upstream tips |
| Defrost the growth factor reduced Matrigel in advance, on ice or in fridge |
| Protocol tips |
| Replace growth factors every other day and Add fresh media every four days. |
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