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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Keep isolated organoids on ice up to 2 h before seeding for 3D culture
Seed 200, 400, or 1000 organoids per dome
Use of 24-well culture plate |
Collect organoids for histology, gene expression, and Western blot analysis |
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Keep isolated organoids on ice up to 2 h before seeding for 3D culture
Seed 200, 400, or 1000 organoids per dome
Use of 24-well culture plate |
| Downstream tips |
| Collect organoids for histology, gene expression, and Western blot analysis |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost in advance basement membrane extract (BME; Cultrex) on ice or in fridge |
Use uncoated 24-well plates
Refresh medium every 2-3 days |
|
| Upstream tips |
| Defrost in advance basement membrane extract (BME; Cultrex) on ice or in fridge |
| Protocol tips |
Use uncoated 24-well plates
Refresh medium every 2-3 days |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Use of an eight-well LabTek chamber
Coat the wells with 60μL of a 60% Matrigel (BD Biosciences) solution in BBM |
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Use of an eight-well LabTek chamber
Coat the wells with 60μL of a 60% Matrigel (BD Biosciences) solution in BBM |
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