3D Cell Culture Media Primary human breast tumors-Mammospheres

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Found 3 matching solutions for this experiment

DMEM/F-12

Thermo Fisher Scientific

Protocol tips
Seed cancer cells at a density of 1000cells/ml
Use of ultra-low adhesion plates
Count mammospheres with diameter > 75μm
MammoCult™ Human Medium Kit

STEMCELL technologies

Protocol tips
Incubation of mammospheres under hypoxia
Passages of mammospheres every 4 days
Count of mammospheres at least 50 μm diameter
Upstream tips
Process tumor specimens 1h of surgery
Protocol tips
Use ultralow attachment plates
Change media every third day
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