Apoptosis assay cell type - A2780

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

Upstream tips
- Prepare 1x Binding Buffer by diluting 1 ml of the 10x Binding Buffer with 9 ml of deionized water.

- Dissolve the staurosporine in DMSO to a concentration of 100 mg/ml
Protocol tips
- 1 × 10^4 cells were collected and stained with Annexin V-FITC
Upstream tips
- 1×10^4 cells/ml were used for the assay.

- Treated with 25 µM myricetin for 48 h at 37°C
Protocol tips
Add 5 µl of PE Annexin V and 5 µl 7-AAD
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms