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Found 6 matching solutions for this experiment
Upstream tips |
Protocol tips |
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- Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time.
- Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment. |
Downstream tips |
- Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time.
- Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment. |
Upstream tips |
Protocol tips |
Downstream tips |
- Lyse cells in RIPA buffer containing 10% phosphatase inhibitor and 10% protease inhibitor cocktail. |
- Dilute primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
- Dilute primary Ab at 1:1000 |
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Upstream tips |
- Lyse cells in RIPA buffer containing 10% phosphatase inhibitor and 10% protease inhibitor cocktail. |
Protocol tips |
- Dilute primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
- Dilute primary Ab at 1:1000 |
Upstream tips |
Protocol tips |
Downstream tips |
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- Dilute primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
- Dilute primary Ab at 1:1000 |
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Protocol tips |
- Dilute primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
- Dilute primary Ab at 1:1000 |
Upstream tips |
Protocol tips |
Downstream tips |
|
- Dilute primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
- Dilute primary Ab at 1:1000 |
|
Protocol tips |
- Dilute primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
- Dilute primary Ab at 1:1000 |
Upstream tips |
Protocol tips |
Downstream tips |
|
- Dilute primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
- Dilute primary Ab at 1:1000 |
|
Protocol tips |
- Dilute primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
- Dilute primary Ab at 1:1000 |
Upstream tips |
Protocol tips |
Downstream tips |
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- Dilute Ab at 1:10000 in blocking solution.
- incubate primary antibodies overnight at 4°C. |
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Protocol tips |
- Dilute Ab at 1:10000 in blocking solution.
- incubate primary antibodies overnight at 4°C. |
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