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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - Lysis can be performed at 4°C in lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100) supplemented with a cocktail of protease inhibitors |
- Incubate primary and secondary antibodies for 1 h at room temperature, or overnight at 4°C. |
|
| Upstream tips |
| - Lysis can be performed at 4°C in lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100) supplemented with a cocktail of protease inhibitors |
| Protocol tips |
| - Incubate primary and secondary antibodies for 1 h at room temperature, or overnight at 4°C. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- Stain with 10 µg/ml of antibody in blocking buffer for 1 h. |
|
| Protocol tips |
| - Stain with 10 µg/ml of antibody in blocking buffer for 1 h. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- Dilute Primary Ab at-1:200 dilution |
|
| Protocol tips |
| - Dilute Primary Ab at-1:200 dilution |
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