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Found 7 matching solutions for this experiment
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Cells were cultured in normal culture medium under normoxia (21% O2) or hypoxia (1% O2) conditions for the indicated times. Cells were subsequently lysed in RIPA lysis buffer containing protease inhibitors and subjected to immunoblotting with primary antibodies |
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| Cells were cultured in normal culture medium under normoxia (21% O2) or hypoxia (1% O2) conditions for the indicated times. Cells were subsequently lysed in RIPA lysis buffer containing protease inhibitors and subjected to immunoblotting with primary antibodies |
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Dilute primary antibody in 1% normal serum or BSA (in 1X PBS). Incubate coverslips with ~5 µ g/mL rabbit anti-LC3 primary antibody (NB100-2220) for 1 hour at room temperature (37°C is optional), or for 16 hours at 4°C. |
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| Dilute primary antibody in 1% normal serum or BSA (in 1X PBS). Incubate coverslips with ~5 µ g/mL rabbit anti-LC3 primary antibody (NB100-2220) for 1 hour at room temperature (37°C is optional), or for 16 hours at 4°C. |
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Incubate cells with both primary antibodies in 1% BSA in PBST in a humidified chamber for 1 h at room temperature or overnight at 4°C. |
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| Incubate cells with both primary antibodies in 1% BSA in PBST in a humidified chamber for 1 h at room temperature or overnight at 4°C. |
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For WB dilute primary Ab between Western Blot 1:500-1:3000 and incubate overnight at 4 °C |
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| For WB dilute primary Ab between Western Blot 1:500-1:3000 and incubate overnight at 4 °C |
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Dilute primary Ab between 1:300-1000 and incubate overnight at 4 °C |
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| Dilute primary Ab between 1:300-1000 and incubate overnight at 4 °C |
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Dilute primary Ab at 1:1000 and incubate overnight at 4°C |
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| Dilute primary Ab at 1:1000 and incubate overnight at 4°C |
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Incubate primary Ab overnight at 4 °C |
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| Incubate primary Ab overnight at 4 °C |
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