Bacterial cell culture media Escherichia coli

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

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Found 4 matching solutions for this experiment

Protocol tips
To prepare plasmids for DNA sequence analysis, the E. coli colony glycerol stocks that yielded positive recombinants according to the PCR colony screens were grown overnight at 37 °C in 2–3 ml of CircleGrow® broth (MP Biomedicals; Santa Ana, CA) in the presence of 50 μg/ml kanamycin.
Protocol tips
E. faecalis was cultured in BHI and the Escherichia coli ST18 donor strain for MPM was cultured in BHI supplemented with δ-aminolevulinic acid (100 µg ml−1).
Upstream tips
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C.
Protocol tips
-Reaction of 2.5% w/v aqueous solution at 25°C. pH : 7.5±0.2
Upstream tips
-Store product at room temperature, keep tightly sealed, and protect from moisture and light.
Protocol tips
-pH (@ 25°C): 7.8 ± 0.2
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