Bacterial cell culture media Pseudomonas aeruginosa

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

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Found 3 matching solutions for this experiment

BRAIN HEART INFUSION BROTH

Thermo Fisher Scientific

Upstream tips
-Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store tubed or bottled medium in the dark and below 20°C.
Protocol tips
Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.
Nutrient agar

Sigma-Aldrich

Upstream tips
Store prepared media below 8°C, protected from direct light. Store dehydrated powder in a dry place in tightly-sealed containers at 2-25°C.
Protocol tips
-Final pH 6.8 +/- 0.2 at 25°C. If desired, the medium can be enriched with 5-10% v/v sterile defibrinated blood.
LB Broth, Lennox (Granulated)

Thermo Fisher Scientific

Protocol tips
-Add 20g to 1L of purified water, such as BP2820 (Fisher BioReagents Microbial Cell Culture Grade Water) then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Before use, sterilize by autoclaving for 15 minutes.
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