Bacterial cell culture media Vibrio cholera

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

Protocol tips
-DO NOT HEAT TO MORE THAN 100 °C. DO NOT AUTOCLAVE AT 121 °C.
- If using an autoclave, do so without pressure.
- 2: In case of samples with a high presence of Aeromonas, 50 mg of cefsulodin can be
added to the mix once cooled down at 45-50 °C (50 mg/L).
-FINAL MEDIA pH 9.0 +/- 0.2
Downstream tips
-Plates can be destroyed by
autoclaving at 121 °C for at least 20 minutes.
Upstream tips
-Store between 15-25°C.
Protocol tips
With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma
capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended.
Protocol tips
-Allow to cool slightly before making additions, such as antibiotics (if desired).
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms