Cell Culture Contamination Detection Kit Bacteria

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

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Found 14 matching solutions for this experiment

Upstream tips
• Antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate).
• The second molecule is labeled with an acceptor fluorophore (ULight™ dye).
• This kit is designed to quantify the levels of DNA (from different hosts, either double- or single-stranded and of varying fragment sizes) in cell culture supernatants.
Protocol tips
• Sensitivity: Lower Detection Limit (LDL): 54.5 pg/mL
• Lower Limit of Quantification (LLOQ): 266.3 pg/mL
• EC50: 50.1 ng/mL
• Dynamic range: Kit designed to detect DNA between: 54.5– 30,000 pg/mL
Upstream tips
• Assay utilizes the polymerase chain reaction (PCR) to amplify a target unique to that microorganism and TaqMan™ probes that identify the specific organism.
• This kit has 3 easy steps: Enrich samples, prepare samples, and run samples.
Protocol tips
• PCR Method: qPCR
• For Use With: 7300 System, 7500 System, AB 7900 HT 384-Well System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode.
• Passive Reference Dye: ROX (Pre-mixed)
Upstream tips
• Staphylococcal food poisoning is caused by eating foods contaminated with enterotoxins produced during the growth of certain strains of Staphylococcus aureus. The enterotoxins A, B, C, D and E play an important role in the pathogenesis of staphylococcal food poisoning which is normally characterised by nausea, vomiting and diarrhoea.
Protocol tips
• The SET-RPLA test kit is based upon the reports by Shingaki et al. and Oda et al.
Upstream tips
• Rapidly identify and differentiate staphylococci which possess coagulase and/or protein A, particularly Staphylococcus aureus, using the Thermo Scientific™ Staphaurex™ Latex Agglutination Test.
Protocol tips
• When mixed on a slide with a suspension of S. aureus, reaction of clumping factor with the fibrinogen and/or protein A with the IgG causes rapid, strong agglutination of the latex particles.
Upstream tips
• The BacTx rapid test is a colorimetric assay that can detect aerobic, anaerobic, gram-negative and gram-positive bacteria in a single test.
Protocol tips
• Up to 8 samples can be processed in parallel in 15 minutes.
• The tubes are placed in the BacTx analyzer, which continuously monitors color development. Test results are displayed and automatically interpreted by the BacTx software.
Upstream tips
• The assay utilizes the PCR to amplify a target unique to that microorganism and TaqMan™ probes that identify the specific organism.
• This kit has 3 easy steps: Enrich samples, prepare samples, and run samples.
Protocol tips
• Hot Start: Built-In Hot Start
• Passive Reference: Dye
ROX (Pre-mixed)
• PCR Method: qPCR
VetMAX™ C. fetus Kit

Thermo Fisher Scientific

Upstream tips
• Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.
Protocol tips
• Detection Method: Primer-Probe Detection
• GC-Rich PCR Performance:
Low
• No. of Reactions: 1 Well
Reaction
VetMAX™ A. phagocytophilum Kit

Thermo Fisher Scientific

Upstream tips
• Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.
Protocol tips
• Detection Method: Primer-Probe Detection
• GC-Rich PCR Performance:
Low
• No. of Reactions: 1 Well
Reaction
Upstream tips
• The workflow utilizes TaqMan™ chemistries and the PCR to detect Salmonella Enteritidis from egg and environmental samples in 27 hours.
Protocol tips
• The egg testing method enables the user to accurately detect down to 1 to 5 cfu of Salmonella Enteritidis from an entire 1L pooled egg enrichment.
Upstream tips
• Three fluorescent dyes distinctly stain yeast (and other fungi), gram-positive and gram negative bacteria by slide preparation.
Protocol tips
• Detection Method: Fluorescent
• The kit provides sufficient material to perform approximately 200 contamination assays
Upstream tips
• For rapid low-level microbial detection even in the presence of PCR inhibitors
• Consistent low-level detection
• Tolerant of PCR inhibitors
Protocol tips
• Reaction Speed: Fast or Standard
• Sample Type: DNA
• Volume: 1 x 50 mL
Upstream tips
• The kit not only detects contaminants, but it also identifies the contaminant type.
• Three fluorescent dyes distinctly stain yeast (and other fungi), gram-positive and gram-negative bacteria by slide preparation.
PET-RPLA Toxin Detection Kit

Thermo Fisher Scientific

Upstream tips
• Detect enterotoxin type A of Clostridium perfringens with Thermo Scientific™ Oxoid™ Clostridium perfringens Enterotoxin type A Reversed Passive Latex Agglutination Kit (PET-RPLA).
Onar® Bacteria

Minerva biolabs

Upstream tips
• The kit can detect more than 45 bacterial genera based on alignment analysis.
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