Cell cycle assay human - HaCaT

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Protocol tips
- Cells are fixed at −20°C for 12 h after washing three times with cold PBS.

- Cells were treated with 200 mg/mL RNase A and 50 μg/mL PI
Protocol tips
- Following seeing, cells were treated with PKF118-310, caperatic acid, physodic acid or camptothecin.

- At −20 °C for at least 24 h cell are fixed with 70% ethanol.
Protocol tips
- Cells were fixed in 70% ethanol overnight.

- To stain cells were resuspended in 500 µl of PI/RNASE staining solution.
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