Cell cycle assay human - MCF 10A

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Cells are fixed in cold 70% ethanol for 3 hours at −20°C.

Protocol tips
- Cells are fixed in cold 70% ethanol for 3 hours at −20°C.

Manufacturer protocol Publication protocol 1 Relevant paper

Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells are labelled with EdU with 10 μM for 40 min

Protocol tips
- Cells are labelled with EdU with 10 μM for 40 min

Manufacturer protocol Publication protocol 1 Relevant paper

APC BrdU Flow Kit

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	Incubate cells with antibodies for 15 minutes on ice.

Protocol tips
Incubate cells with antibodies for 15 minutes on ice.

Manufacturer protocol Publication protocol 1 Relevant paper

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