Cell cycle assay human - OVCAR-5

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

APC BrdU Flow Kit

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	Cells are treated with 0.2 μM TPT, 0.5 μM PF477736, or both incubated for 16 hours. Cells were incubated for 30min after adding BrdU.

Protocol tips
Cells are treated with 0.2 μM TPT, 0.5 μM PF477736, or both incubated for 16 hours. Cells were incubated for 30min after adding BrdU.

Manufacturer protocol Publication protocol 1 Relevant paper

Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells were stained and incubated for 45 min at 37 °C with 10 μg/ml of propidium iodide and 5 μg/ml of ribonuclease A in PBS.

Protocol tips
- Cells were stained and incubated for 45 min at 37 °C with 10 μg/ml of propidium iodide and 5 μg/ml of ribonuclease A in PBS.

Manufacturer protocol Publication protocol 1 Relevant paper

Guava Cell Cycle Reagent for Flow Cytometry

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
- Cells were analysed for cell cycle after 72h

Upstream tips
- Cells were analysed for cell cycle after 72h

Manufacturer protocol Publication protocol 1 Relevant paper

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