Cell cycle assay human - PANC-1

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells were labelled with 10 μmol/L of 5-ethynyl-2′-deoxyuridine and incubated for 60 minutes at 37°C. - Cells were fixed with 4% paraformaldehyde for 60 minutes instead of 15min. - 7-AAD was added for measuring DNA content and cell cycle distribution.

Protocol tips
- Cells were labelled with 10 μmol/L of 5-ethynyl-2′-deoxyuridine and incubated for 60 minutes at 37°C. - Cells were fixed with 4% paraformaldehyde for 60 minutes instead of 15min. - 7-AAD was added for measuring DNA content and cell cycle distribution.

Manufacturer protocol Publication protocol 1 Relevant paper

Cell Cycle and Apoptosis Analysis Kit

Beyotime

Upstream tips	Protocol tips	Downstream tips
	- Cells are fixed with 70% ethanol at 4°C for 24 h. - Cells are stained with 50 μg/mL of PI and 100 μg/mL of RNase A at 37°C for 30 min in the dark.

Protocol tips
- Cells are fixed with 70% ethanol at 4°C for 24 h. - Cells are stained with 50 μg/mL of PI and 100 μg/mL of RNase A at 37°C for 30 min in the dark.

Manufacturer protocol Publication protocol 1 Relevant paper

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- If low level of staining is noticed, increase volume of stain used.

Protocol tips
- If low level of staining is noticed, increase volume of stain used.

Manufacturer protocol Publication protocol 1 Relevant paper

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