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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - Cells were seeded at a cell density of 1×105 cells/ml. |
- Seeded cells were treated with insulin (25 nM) for 24h.
- Cells were incubated with BrdU for 24 h. |
- The absorbance was measured at a dual wavelength of 450–540 nm |
| Upstream tips |
| - Cells were seeded at a cell density of 1×105 cells/ml. |
| Protocol tips |
- Seeded cells were treated with insulin (25 nM) for 24h.
- Cells were incubated with BrdU for 24 h. |
| Downstream tips |
| - The absorbance was measured at a dual wavelength of 450–540 nm |
| Upstream tips |
Protocol tips |
Downstream tips |
| - Cells were plated at a density of 1000 or 2000 cells per well in 96-well plates. |
- cells were incubated in 0.1% CV for 30 min RT |
- If the readings are low return the plate to the dark for longer incubation. |
| Upstream tips |
| - Cells were plated at a density of 1000 or 2000 cells per well in 96-well plates. |
| Protocol tips |
| - cells were incubated in 0.1% CV for 30 min RT |
| Downstream tips |
| - If the readings are low return the plate to the dark for longer incubation. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- Cells were incubated for 3 h at 37 °C after addition of CCK-8 reagent |
|
| Protocol tips |
| - Cells were incubated for 3 h at 37 °C after addition of CCK-8 reagent |
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