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Found 10 matching solutions for this experiment
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purified B cells (>95%) were isolated using MagCellect Human B Cell Isolation Kit (R&D systems, USA). B cell purity was assessed by flow cytometry using APC labeled CD19 antibody (Biolegend, USA). |
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| purified B cells (>95%) were isolated using MagCellect Human B Cell Isolation Kit (R&D systems, USA). B cell purity was assessed by flow cytometry using APC labeled CD19 antibody (Biolegend, USA). |
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During positive selection, the cell type of interest (in this case B-cells) are specifically targeted, labelled and actively magnetically removed from the sample by retention in the column in the presence of a magnetic field. These cells are then retrieved by plunging the column in the absence of a magnetic field. |
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| During positive selection, the cell type of interest (in this case B-cells) are specifically targeted, labelled and actively magnetically removed from the sample by retention in the column in the presence of a magnetic field. These cells are then retrieved by plunging the column in the absence of a magnetic field. |
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B cells were purified from mouse spleen or human PBMC using magnetic negative selection kits (StemCell Technologies). |
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| B cells were purified from mouse spleen or human PBMC using magnetic negative selection kits (StemCell Technologies). |
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HCL and HCLv cells for microarray studies were obtained in sodium heparin tubes and purified by Ficoll centrifugation, followed by total B-cell isolation using the Dynabeads™ Untouched™ Human B Cells Kit (ThermoFisher Scientific). |
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| HCL and HCLv cells for microarray studies were obtained in sodium heparin tubes and purified by Ficoll centrifugation, followed by total B-cell isolation using the Dynabeads™ Untouched™ Human B Cells Kit (ThermoFisher Scientific). |
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CLL cells were negatively selected from fresh blood samples using the RosetteSepTM B cell enrichment cocktail (StemCell Technologies, Grenoble, France) and density gradient centrifugation (Ficoll®Paque Plus, GE Healthcare Life sciences, Velizy-Villacoublay, France). |
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| CLL cells were negatively selected from fresh blood samples using the RosetteSepTM B cell enrichment cocktail (StemCell Technologies, Grenoble, France) and density gradient centrifugation (Ficoll®Paque Plus, GE Healthcare Life sciences, Velizy-Villacoublay, France). |
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Human CD43− resting B cells were isolated from PBMCs using MojoSort Human B Cell (CD43−) Isolation Kit (BioLegend), according to the manufacturer's protocols. |
Isolated resting B cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 100 μg/ml streptomycin, 100 U/ml penicillin, and 1 mM 2-mercaptoethanol. |
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| Human CD43− resting B cells were isolated from PBMCs using MojoSort Human B Cell (CD43−) Isolation Kit (BioLegend), according to the manufacturer's protocols. |
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| Isolated resting B cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 100 μg/ml streptomycin, 100 U/ml penicillin, and 1 mM 2-mercaptoethanol. |
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| For positive selection, an aliquot of 10×106 frozen PBMCs was used for sequential isolations of B cells followed by CD8+ T cells and another 10x106 frozen PBMC aliquot was used for sequential isolation of monocytes followed by CD4+ T cells. |
according to manufacturer's instructions. |
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| For positive selection, an aliquot of 10×106 frozen PBMCs was used for sequential isolations of B cells followed by CD8+ T cells and another 10x106 frozen PBMC aliquot was used for sequential isolation of monocytes followed by CD4+ T cells. |
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| according to manufacturer's instructions. |
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| For positive selection, an aliquot of 10×106 frozen PBMCs was used for sequential isolations of B cells followed by CD8+ T cells and another 10x106 frozen PBMC aliquot was used for sequential isolation of monocytes followed by CD4+ T cells. |
according to manufacturer's instructions. |
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| For positive selection, an aliquot of 10×106 frozen PBMCs was used for sequential isolations of B cells followed by CD8+ T cells and another 10x106 frozen PBMC aliquot was used for sequential isolation of monocytes followed by CD4+ T cells. |
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| according to manufacturer's instructions. |
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Following this B cells were enriched by magnetic microparticle purification using the EasySep Human B cell Enrichment Kit Without CD43 Depletion. |
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| Following this B cells were enriched by magnetic microparticle purification using the EasySep Human B cell Enrichment Kit Without CD43 Depletion. |
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Primary B-cells were purified from peripheral blood using EasySep™ Direct Human B Cell Isolation Kit from Stemcells Technologies Cat #19674 and directly used for RNA purification. |
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| Primary B-cells were purified from peripheral blood using EasySep™ Direct Human B Cell Isolation Kit from Stemcells Technologies Cat #19674 and directly used for RNA purification. |
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