Cell line authentication Colon cancer cell line HCT-116

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Found 2 matching solutions for this experiment

Upstream tips
Extract total genomic DNA was using the PureLink® Genomic DNA Mini Kit (K1820-02, Invitrogen)
Protocol tips
Follow the manufacturer’s instructions
Downstream tips
For data analysis: analyze the STR data using the DSMZ (German Collection of Microorganisms and Cell Cultures) online STR database
Upstream tips
Isolate DNA using phenol/chloroform, or magnetic beads (the Maxwell 16 DNA Purification kits, Promega), and MagAttract DNA Mini M48 kit, Qiagen)
Protocol tips
Match STR profiles with the ATCC's and German Collection of Microorganisms and Cell cultures' (Braunschweig, Germany) online databases
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