Cell line authentication Renal carcinoma cell line Caki-1

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Found 2 matching solutions for this experiment

Upstream tips
Extract total genomic DNA was using the PureLink® Genomic DNA Mini Kit (K1820-02, Invitrogen)
Protocol tips
Follow the manufacturer’s instructions
Downstream tips
For data analysis: analyze the STR data using the DSMZ (German Collection of Microorganisms and Cell Cultures) online STR database
Upstream tips
Extract DNA from cell pellets using QiaAMP mini preps (Qiagen cat 51306)
Protocol tips
Use of a DNA amount of 0.15 ng, so that lower-level mixing of cell lines could be visible
Compare the STR profiles to known ATCC fingerprints (ATCC.org), to the Cell Line Integrated Molecular Authentication database
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