Cell migration / Invasion cell type - PC-3

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 5 matching solutions for this experiment

QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
Seed 0.5 × 10^6 to 1 × 10^6 cells	Add 10% FBS complete medium in the lower chamber. Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).	Read plate at 570nm

Upstream tips
Seed 0.5 × 10^6 to 1 × 10^6 cells
Protocol tips
Add 10% FBS complete medium in the lower chamber. Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).
Downstream tips
Read plate at 570nm

Manufacturer protocol Publication protocol 1 Relevant paper

ORIS™ Cell Migration Assay

Platypus Technologies

Upstream tips	Protocol tips	Downstream tips
	Incubate cells in a humidified chamber (37°C, 5% CO2) for 4 to 18 hours (cell line dependent) to permit cell attachment.	To read use 485/528 nm excitation/emission filters

Protocol tips
Incubate cells in a humidified chamber (37°C, 5% CO2) for 4 to 18 hours (cell line dependent) to permit cell attachment.
Downstream tips
To read use 485/528 nm excitation/emission filters

Manufacturer protocol Publication protocol 1 Relevant paper

Cultrex® Cell Migration Assay

Trevigen

Upstream tips	Protocol tips	Downstream tips
	In the lower chnaber add medium supplemented with 10% FBS. Incubate plates at 37 °C (5% v/v CO2) for 24 h,	Read plate at 485 nm excitation, 520 nm emission

Protocol tips
In the lower chnaber add medium supplemented with 10% FBS. Incubate plates at 37 °C (5% v/v CO2) for 24 h,
Downstream tips
Read plate at 485 nm excitation, 520 nm emission

Manufacturer protocol Publication protocol 1 Relevant paper

Cultrex® BME Cell Invasion Assay

Bio-Techne

Upstream tips	Protocol tips	Downstream tips
	In the lower chnaber add medium supplemented with 10% FBS. Incubate plates at 37 °C (5% v/v CO2) for 24 h,	Read plate at 485 nm excitation, 520 nm emission

Protocol tips
In the lower chnaber add medium supplemented with 10% FBS. Incubate plates at 37 °C (5% v/v CO2) for 24 h,
Downstream tips
Read plate at 485 nm excitation, 520 nm emission

Manufacturer protocol Publication protocol 1 Relevant paper

CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
Suspend 100 000 cells/mL	Incubate for 72 hours in a cell culture incubator.

Upstream tips
Suspend 100 000 cells/mL
Protocol tips
Incubate for 72 hours in a cell culture incubator.

Manufacturer protocol Publication protocol 1 Relevant paper

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