DNA Damage Assay A-375

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

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Found 2 matching solutions for this experiment

Upstream tips
Seed 1–2 × 10^4 cells/ml
Downstream tips
Corresponding TIFF files were processed using the ImageJ plugin Open Comet (http://www.cometbio.org/), and EdU+ cells were manually scored for each cell ID generated.
Comet Assay

Sigma-Aldrich

Upstream tips
Seed 2X10^5cells/ml
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