DNA Damage Assay HeLa

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 4 matching solutions for this experiment

Upstream tips
Cell suspension at a concentration of 10^5cells/ml was mixed in a 1:1 ratio withTrevigen LMAgarose (#4250-050-02) at 37◦C
Downstream tips
The images of the comets can be analyzed using Cell Profiler software (version 2.1.1 rev 6c2d896).
Upstream tips
Seed 1x10^5 cells/ml/well
Downstream tips
Processed samples can be viewed and captured under the ZEISS® Axio Vert.A1 Microscope. Percentage DNA tail (%) and tail length of comets can be obtained using the OpenComet software .
Upstream tips
Seed 2 × 10^5 cells/m/well
Downstream tips
Use TriTek Comet Image program to analyze the fluorescence images to circumscribe the “head” and the “tail” regions of each comet.
Upstream tips
Seed 2 × 10^5 cells/ml
Downstream tips
Use TriTek Comet Image program to analyze the fluorescence images to circumscribe the “head” and the “tail” regions of each comet.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms