DNA Damage Assay HT1080

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

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Found 3 matching solutions for this experiment

Upstream tips
Cell suspension at a concentration of 10^5cells/ml was mixed in a 1:1 ratio withTrevigen LMAgarose (#4250-050-02) at 37◦C.
Downstream tips
Use Cell Profiler software (version 2.1.1 rev 6c2d896) for analyzing the images of comet.
Upstream tips
Seed 2X10^5 cells/ml
Downstream tips
The visualized cell images can be analyzed by the automated tool for comet assay, OpenComet.
Upstream tips
Seed × 10^5 cells/ml/well
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