DNA Damage Assay MDA-MB-231

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

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Found 2 matching solutions for this experiment

Comet Assay Kits, 96-Well

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
Resuspend the cells at 1 x 10^5 cells/mL in ice-cold PBS (without Mg2+ and Ca2+).		Comet tail lengths can be quantified by NIH ImageJ software. Each data point can be calculated as an average of 100 cells,

Upstream tips
Resuspend the cells at 1 x 10^5 cells/mL in ice-cold PBS (without Mg2+ and Ca2+).
Downstream tips
Comet tail lengths can be quantified by NIH ImageJ software. Each data point can be calculated as an average of 100 cells,

Manufacturer protocol Publication protocol 1 Relevant paper

CometAssay Single Cell Gel Electrophoresis Assay

Bio-Techne

Upstream tips	Protocol tips	Downstream tips
Seed 1–2 × 10^4 cells/ml	Procedure for alkaline comet assay

Upstream tips
Seed 1–2 × 10^4 cells/ml
Protocol tips
Procedure for alkaline comet assay

Manufacturer protocol Publication protocol 1 Relevant paper

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