DNA Damage Assay U266

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

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Found 2 matching solutions for this experiment

Upstream tips
Seed 2X10^5 cells/ml
Protocol tips
These experiments were repeated at lower density cell concentrations (2x10^5), and incubation times extended for 4, 8, 16, 24, and 48 hours to see how the crosslinks were repaired over time (n¼4).
Upstream tips
Seed 2x10^6/mL cells / well
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