DNA Ladder 1 kb

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

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Found 22 matching solutions for this experiment

Upstream tips
The range of the ladder is 0.5 kb to 48.5 kb
Protocol tips
It's recommended to load 10 μl (0.5 μg) of Quick-Load 1 kb Extend DNA Ladder per gel lane. For optimum separation of the highest molecular weight DNA fragments, run on a 0.6% (1X TAE or 1X TBE) agarose gel at 3.5 V/cm (distance between electrodes) for 3 hours.
Upstream tips
Fluorescent 1 kb DNA Ladder consists of 10 DNA fragments ranging from 1 kb to 10 kb
Protocol tips
DNA Ladders with Fluorescent Stain are optimized for direct loading into unstained agarose Recommended Load:
5 μl per lane/gels and are recommended for use in combination with Gel Loading Buffer with DNA Stain
Upstream tips
The range of the ladder is 100 bp to 10 kb
Protocol tips
For GelRed / GelGreen Precast Gels: Load 5 µl (0.25 µg) per gel lane
For SYBR Safe Precast Gels: Load 10 µl (0.5 µg) per gel lane
Recommended gel percentage range: 1-1.4%
1 kb DNA Ladder

New England BioLabs

Upstream tips
The range of the ladder is 0.5-10.0 kilobases (kb)
Protocol tips
Recommended Load Dilutions:
Distilled water (dH20)* or TE Buffer- 4 μl
Gel Loading Dye, Purple (6X), no SDS- 1 μl
DNA Ladder/Marker- 1 μl
Total volume- 6 μl
Upstream tips
The range of the ladder is 100 bp to 10 kb
Protocol tips
Load 5–10 µl (0.5–1.0 µg) of Quick-Load 1 kb Plus DNA Ladder per gel lane
Recommended gel percentage range: 1-1.4%
Upstream tips
Size Range: 250bp - 10,000 bp
Protocol tips
Recommended Load: 6ul/well
The ladder is supplied in a ready-to-use format containing fluorescent DNA stain and tracking dyes.
Quick-Load® 1 kb DNA Ladder

New England BioLabs

Upstream tips
The range of the ladder is 500 bp to 10 kb
Protocol tips
Load 10 μl (0.5 μg) of Quick-Load 1 kb DNA Ladder per gel lane
Recommended gel percentage range: 0.8-1.2%
TriDye™ 1 kb DNA Ladder

New England BioLabs

Upstream tips
The range of the ladder is 500 bp to 10 kb
Protocol tips
Load 10 μl (0.5 μg) of TriDye 1 kb DNA Ladder per gel lane
Recommended gel percentage range: 0.8-1.2%
Upstream tips
The range of the ladder is 100 bp to 10 kb
Protocol tips
Load 5-10 µl (0.5-1 µg) of Quick-Load Purple 1kb Plus DNA Ladder per gel lane
Upstream tips
The range of the ladder is 250bp to 10,000bp
Protocol tips
1 kb DNA ladder is compatible with 1% standard and precast agarose slab gels.
1 kb Plus DNA Ladder

New England BioLabs

Upstream tips
Recommended load: 0.5-1 μg of the 1 kb Plus DNA Ladder diluted in sample buffer
Protocol tips
The range of the ladder is 100bp-10kb
Upstream tips
The range of the ladder is 100 bp to 10 kb
Protocol tips
Load 5-10 μl (0.5-1.0 μg) of TriDye 1 kb Plus DNA ladder per gel lane
Upstream tips
The range of the ladder is 500bp to 10kb
Protocol tips
Load 10 µl (0.5 µg) of Quick-Load Purple 1 kb DNA Ladder per gel lane
DNA Ladder & Loading Dye

STEMCELL technologies

Upstream tips
Size range: 250 bp to 10,000 bp
Protocol tips
The recommended loading volume of 1 kb DNA Ladder is 6 μL per lane
E-Gel™ 1 Kb Plus DNA Ladder

Thermo Fisher Scientific

Upstream tips
The range of the ladder is 100-15,000 bp
Protocol tips
Load equal amount of DNA ladder and DNA sample
TrackIt™ 1 Kb Plus DNA Ladder

Thermo Fisher Scientific

Upstream tips
The range of the ladder is 100-15,000 bp
Protocol tips
Load equal amount of DNA ladder and DNA sample
Upstream tips
The range of the ladder is 100-5,000bp
Protocol tips
Load equal amount of DNA ladder and DNA sample
Upstream tips
The range of the ladder is 1kb to 10kb in 1kb increments
Protocol tips
Recommended Load: 1ul / lane
Upstream tips
The range of the ladder is 250bp to 3000kb
Protocol tips
Recommended Load: 5 ul / lane
Upstream tips
The range of the ladder is 250bp to 3000kb
Protocol tips
Recommended Load: 6 ul / lane
Upstream tips
The range of the ladder is 100-10,000 bp
Protocol tips
Recommended Load: 5 ul / well
1 Kb DNA Ladders

Xcelris Genomics

Upstream tips
Volume: 1250 µl
Applications: 250
Loading Volume: 5 µl
No of Bands: 8
Band size:500bp, 1000bp, 2000bp, 3000bp, 4000bp, 500bp, 6000bp, 8000bp
Protocol tips
Storage: at 4°C for 30 days; at -20°C for two year
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