DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

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Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.
Protocol tips
Ensure DNA is mixed thoroughly prior to application. The wells of
the assay strip bind up to 200 ng of total DNA.
Protocol tips
Ensure DNA is mixed thoroughly prior to application. The wells of
the assay strip bind up to 200 ng of total DNA.
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