ELISA Human - FABP2

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

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Found 3 matching solutions for this experiment

Human FABP2/I-FABP DuoSet ELISA

R&D Systems

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.	-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Upstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.
Protocol tips
-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Manufacturer protocol Publication protocol 1 Relevant paper

I-FABP, Human, ELISA kit

Hycult Biotech

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. -Do not include sodium azide in buffers or wash solutions if an HRP-labeled antibody/conjugate will be used for detection.	-Standards (duplicates or triplicates) and blank must be run with each plate to ensure accuracy. -Use a humidified incubator to reduce drying effects in the outside wells (“edge effects”) or place a wet tissue or blotter paper under the plates in non-humidified incubators. -Do not stack plates.

Upstream tips
-Bring all reagents to room temperature before use. -Do not include sodium azide in buffers or wash solutions if an HRP-labeled antibody/conjugate will be used for detection.
Protocol tips
-Standards (duplicates or triplicates) and blank must be run with each plate to ensure accuracy. -Use a humidified incubator to reduce drying effects in the outside wells (“edge effects”) or place a wet tissue or blotter paper under the plates in non-humidified incubators. -Do not stack plates.

Manufacturer protocol Publication protocol 1 Relevant paper

Human FABP2 / Fatty Acid-Binding Protein, Intestinal ELISA Kit

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents and samples to room temperature (18–25 °C) before use.	-Wash by filling each well with Wash Buffer using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. -Read at 450 nm immediately after adding STOP solution.

Upstream tips
-Bring all reagents and samples to room temperature (18–25 °C) before use.
Protocol tips
-Wash by filling each well with Wash Buffer using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. -Read at 450 nm immediately after adding STOP solution.

Manufacturer protocol Publication protocol 1 Relevant paper

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