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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution. |
|
| Upstream tips |
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. |
| Protocol tips |
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -All reagents are stable as supplied at 4°C, except the Recombinant HO-1 Standard, which should be stored at -20°C. For optimum storage, the Recombinant HO-1 Standard should be aliquotted into smaller portions and stored at -20°C. |
-Include a standard curve each time the assay is performed.
-Mix all reagents and samples gently, yet thoroughly, prior to use. Avoid foaming of reagents.
-In this protocol, room temperature refers to 20-28C. The room temperature should remain within this range throughout the assay.
-Consistent, thorough washing of each well is critical.
|
|
| Upstream tips |
| -All reagents are stable as supplied at 4°C, except the Recombinant HO-1 Standard, which should be stored at -20°C. For optimum storage, the Recombinant HO-1 Standard should be aliquotted into smaller portions and stored at -20°C. |
| Protocol tips |
-Include a standard curve each time the assay is performed.
-Mix all reagents and samples gently, yet thoroughly, prior to use. Avoid foaming of reagents.
-In this protocol, room temperature refers to 20-28C. The room temperature should remain within this range throughout the assay.
-Consistent, thorough washing of each well is critical.
|
| Upstream tips |
Protocol tips |
Downstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
| Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
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