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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment.
-Avoid repeated freeze-thaw cycles.
-The kit may be stored at 4°C for up to 6 months.
-Bring all reagents and samples to room temperature (18 - 25ºC) before use. |
-Levels of Dkk-1 may vary between different samples.
Optimal dilution factors
for each sample must be determined by the investigator.
-A standard curve must be run with each assay.
-Keep substrate solution protected from light.
-Read immediately after adding STOP solution. |
-Store your standard at <-70ºC after reconstitution, others at 4ºC. |
| Upstream tips |
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment.
-Avoid repeated freeze-thaw cycles.
-The kit may be stored at 4°C for up to 6 months.
-Bring all reagents and samples to room temperature (18 - 25ºC) before use. |
| Protocol tips |
-Levels of Dkk-1 may vary between different samples.
Optimal dilution factors
for each sample must be determined by the investigator.
-A standard curve must be run with each assay.
-Keep substrate solution protected from light.
-Read immediately after adding STOP solution. |
| Downstream tips |
| -Store your standard at <-70ºC after reconstitution, others at 4ºC. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
|
| Upstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
| Protocol tips |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
| Upstream tips |
| -Equilibrate all reagents to room temperature (18-25°C) prior to use. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
| Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C. |
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