Mammalian cell culture media A253

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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McCoy's 5A Medium, ATCC® 30-2007™

VWR

Upstream tips	Protocol tips	Downstream tips
	The A253 cell line was purchased from ATCC and grown in McCoy’s 5a media (also sourced from ATCC), per supplier’s guidance. Primary salivary hS/PCs were obtained as previously described (Pradhan-Bhatt et al. 2013, 2014; Srinivasan et al. 2017) and grown in Hepatocyte Defined media (Discovery Labware) containing epidermal growth factor at a concentration of 5 μg/500 mL. Cells in passages 5 to 7 were used for all experiments.

Protocol tips
The A253 cell line was purchased from ATCC and grown in McCoy’s 5a media (also sourced from ATCC), per supplier’s guidance. Primary salivary hS/PCs were obtained as previously described (Pradhan-Bhatt et al. 2013, 2014; Srinivasan et al. 2017) and grown in Hepatocyte Defined media (Discovery Labware) containing epidermal growth factor at a concentration of 5 μg/500 mL. Cells in passages 5 to 7 were used for all experiments.

Protocol tips

The A253 cell line was purchased from ATCC and grown in McCoy’s 5a media (also sourced from ATCC), per supplier’s guidance. Primary salivary hS/PCs were obtained as previously described (Pradhan-Bhatt et al. 2013, 2014; Srinivasan et al. 2017) and grown in Hepatocyte Defined media (Discovery Labware) containing epidermal growth factor at a concentration of 5 μg/500 mL. Cells in passages 5 to 7 were used for all experiments.

Manufacturer protocol Publication protocol 1 Relevant paper

Dulbecco’s Modified Eagle’s Medium (DMEM)

Welgene

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (Welgene) and 1% penicillin/streptomycin (Gibco, Carlsbad, CA, USA) at 37°C in a humidified 5% CO2 incubator.

Protocol tips
supplemented with 10% fetal bovine serum (Welgene) and 1% penicillin/streptomycin (Gibco, Carlsbad, CA, USA) at 37°C in a humidified 5% CO2 incubator.

Manufacturer protocol Publication protocol 1 Relevant paper

Gibco™ RPMI 1640 Medium

Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (GIBCO-BRL, Life Technologies).

Protocol tips
supplemented with 10% fetal bovine serum (GIBCO-BRL, Life Technologies).

Manufacturer protocol Publication protocol 1 Relevant paper

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