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Found 3 matching solutions for this experiment
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The cells were cultured and maintained in endothelial cell medium (ECM; cat. no. 1001; ScienCell Research Laboratories, Inc., Carlsbad, CA, USA) containing 5% fetal bovine serum, 1% endothelial cell growth supplement and 1% penicillin/streptomycin at 37°C in a humidified incubator (5% CO2). The experimental cells were treated with BTXA (0.1, 0.2, 0.4, 0.8, 1.6 or 3.2 U/ml) for 12 h before induction of hypoxia. Control cells were treated with PBS for the same period of time. |
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| The cells were cultured and maintained in endothelial cell medium (ECM; cat. no. 1001; ScienCell Research Laboratories, Inc., Carlsbad, CA, USA) containing 5% fetal bovine serum, 1% endothelial cell growth supplement and 1% penicillin/streptomycin at 37°C in a humidified incubator (5% CO2). The experimental cells were treated with BTXA (0.1, 0.2, 0.4, 0.8, 1.6 or 3.2 U/ml) for 12 h before induction of hypoxia. Control cells were treated with PBS for the same period of time. |
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The cell suspension was cultured in cell culture plates with endothelial (EC) culture basal medium (EGM-2, Lonza, USA) containing 2% of FBS (Lonza, USA), 10 units ml−1 of penicillin, 10 μg ml−1 of streptomycin (1% of P/S solution, Lonza, USA) and endothelial growth supplement (Lonza, USA). Incubation was carried out in a humidified atmosphere of 95% air and 5% CO2 at 37 °C. |
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| The cell suspension was cultured in cell culture plates with endothelial (EC) culture basal medium (EGM-2, Lonza, USA) containing 2% of FBS (Lonza, USA), 10 units ml−1 of penicillin, 10 μg ml−1 of streptomycin (1% of P/S solution, Lonza, USA) and endothelial growth supplement (Lonza, USA). Incubation was carried out in a humidified atmosphere of 95% air and 5% CO2 at 37 °C. |
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Culture the HMEC-1 (ATCC CRL-3243) cells at 37 °C in a 7% CO2 humidified atmosphere using MCDB-131 medium with phenol red and supplemented with 15% fetal calf serum, penicillin, streptomycin, L-glutamine 10 mM, hydrocortisone 1 μg/mL, endothelial cell growth supplement (20 μg/mL). |
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| Culture the HMEC-1 (ATCC CRL-3243) cells at 37 °C in a 7% CO2 humidified atmosphere using MCDB-131 medium with phenol red and supplemented with 15% fetal calf serum, penicillin, streptomycin, L-glutamine 10 mM, hydrocortisone 1 μg/mL, endothelial cell growth supplement (20 μg/mL). |
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