Mammalian cell culture media HCtASMC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 2 matching solutions for this experiment

SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM

Lonza

Upstream tips	Protocol tips	Downstream tips
	Experimental details for studies in primary human carotid artery smooth muscle cells (SMCs) and endothelial cells (HCtASMCs and human carotid artery endothelial cells; Cell Applications), using the Streamer apparatus (Flexcell International Corporation, Burlington, VT) with adjustable flow and shear stress conditions are described in the Methods in the Online Data Supplement.

Protocol tips
Experimental details for studies in primary human carotid artery smooth muscle cells (SMCs) and endothelial cells (HCtASMCs and human carotid artery endothelial cells; Cell Applications), using the Streamer apparatus (Flexcell International Corporation, Burlington, VT) with adjustable flow and shear stress conditions are described in the Methods in the Online Data Supplement.

Manufacturer protocol Publication protocol 1 Relevant paper

Human MesoEndo Cell Growth Medium

Cell Applications Inc

Upstream tips	Protocol tips	Downstream tips
	Cells were cultured up to passages 5 through 8 in subtype-specific growth medium (Cell Applications). Cells were seeded on 6-, 12-, or 24-well plates and treated or radiated at a density of 70% to 80%. Endothelial cells were cultured and seeded in gelatin-coated (Attachment Factor Protein 1X, Thermo Fisher Scientific) flasks and plates.

Protocol tips
Cells were cultured up to passages 5 through 8 in subtype-specific growth medium (Cell Applications). Cells were seeded on 6-, 12-, or 24-well plates and treated or radiated at a density of 70% to 80%. Endothelial cells were cultured and seeded in gelatin-coated (Attachment Factor Protein 1X, Thermo Fisher Scientific) flasks and plates.

Manufacturer protocol Publication protocol 1 Relevant paper

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