Mammalian cell culture media HCF

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Protocol tips
To mimic effects of inflammation, cells were incubated with LPS 10 ng/ml and control cells incubated without LPS, for 24 h.
Protocol tips
cultured in FGM-3 medium, in a humidified incubator at 90% N2, 5% O2, 5% CO2 at all times unless otherwise indicated
DMEM/F-12

Thermo Fisher Scientific

Protocol tips
DMEM:F12 supplemented with 10% human serum, penicillin/streptomycin
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